Year: 2018 | Month: February | Volume 8 | Issue 1
Study of Phytochemical and Immunomodulatory Activity of Methanolic Extract of Andrographis Paniculata in Broiler Birds
Abstract:
This study was conducted to investigate the phytochemical screening and immunomodulatory action of methanolic extract of Andrographis Paniculata in experimental model of immunity as alternative to antibiotic growth promoter in broiler chicks. Extracts of plant were obtained by soxhlets extraction in solvent methanol. In this study, a total 60 day old Ven Cobb broiler chicks were used. For cell mediated immunity study, 30 chicks were divided into 3 treatment groups (T1, T2 and T3) 10 chicks in each replicates. Similarly, for humoral immunity study, 30 chicks were divided into another 3 treatment groups of 10 chicks in each replicates. Each bird of different groups was individually identified by using leg band. T1 (Control diet), T2 (Standard growth promoter; BMD @ 0.05% in feed), T3 (APE @ 0.4g/L) in drinking water daily for consecutive 42 days. The preliminary phytochemical tests were carried out by using standard methods. Cellular immunity assessed by DNFB skin sensitization test. Whereas, humoral immunity was analyzed by indirect micro haemagglutination test. Phytochemical analysis revealed the presence of alkaloids, glycosides, phytosterols, diterpenoid, tannin, saponin, flavonoids and phenols. Andrographis paniculta extract in group T3 showed highest antibody titer and significantly higher skin thickness in DNFB skin sensitization test both at 24 hours and 48 hours after sensitization as compared to group T1 and group T2. Phytochemical compounds phenols and flavonoids are known to have beneficial importance in immunomodulation. It can be concluded that the Andrographis paniculata extract posses potent immunomodulatory activity.
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